Kit components In a statement Single packaging × 25 per single package containing: ZS-cTnI-S-1 reactor A plastic straw A desiccant packet
Reactor Stability The original reactor at room temperature (2 ° C to 25 ° C) storage. See the period of validity from kit label.
Steps Before the operation carefully read manuals. 1. Remove packaging from a single reactor. Detection devices will be reflected on the stage. Available to room temperature. 2. When necessary, use distilled water to dilute the positive standard freeze-dried to HepG2, and then used to dilute negative serum concentration of low-standard goods, negative standard without dilution. 3. Samples will be available to room temperature or standards. 4.80 lessons with a plastic straw μ L ~ 100 serum samples of fresh or standard, by adding reactor hole in the sample.
Results Sample after 10 to 15 minutes to read the result. Over time to read the results of a false positive risk. Serum samples of high-viscosity, and in the background clear response after reading the results. Positive: A detection zone in the line of control zone in a clear line of control (= 2 red line that cTnI sample concentration> 0.3). Negative: Not a testing line, with only the control line (= control a red line that cTnI concentrations below detection kit for the critical value)
Through a series of sample results judgment, we can see that with the concentration of serum samples cTnI increase in the intensity of band increased. If students Lantier cTnI reactor and the results of other methods and symptoms consistent with the patient, that patients with myocardial infarction. When patients with suspected myocardial infarction, it is recommended to patients at different time from the series of samples done. If the result is negative and with the results of other methods not at the same time, there is a need for re-determination after the first hour. If the result remains negative, and samples used by patients in the sample, the incidence has more than six hours, unlike patients with myocardial infarction. Generally positive results in a few hours or days remain unchanged. If the results need archiving, laboratory safety can be called chromatography removed from the reactor after preservation
Sensitivity When the standard cTnI soluble in human serum concentrations greater than 0.3ng/ml, use of the reactor Lantier cTnI can be detected positive results. Specificity Installation of this reaction by skeletal muscle calcium containing eggs (sTnI), troponin C (Mi) and troponin T (cTnT), serum, no cross-reaction.
Note 1, the kit is only used in vitro measurement of serum cardiac troponin I (cTnI). Reaction devices and processing specimens in accordance with the general rules and laboratory attention. During the testing within 10 to 15 minutes should not touch the reactor. 2. Every reactor in just one. Proofreading batches before use and validity. Do not use outdated equipment and the standard reaction. The use of packaging is not damaged reactor. If a batch reactor with a positive control line, the reactor can not be used for diagnosis 3, in order to avoid a false positive result, the need fresh blood sample within three hours of use. Needless to temporarily take in the sample after sample preparation immediately frozen, use complex to melt at room temperature before using. Second samples can freeze-thaw. 4. In the final diagnosis, not to rely solely on a certain test results, patients should combine the integrated considered. 5. The new batch of kits before use, or other circumstances so warrant, to preferences for quality control testing